Tg(KRT14-MAP2K1/Esr1)12Pkha

The transgene contains a 2075-bp human keratin 14 promoter construct with a 5' intron from a beta-globin gene driving expression of a cDNA that encodes a constitutively active mutant human mitogen activated protein kinase 1 (with deletion of amino acids 32-51, which comprise a putative alpha-helical domain, and the amino acid substitutions Ser218Glu and Ser222Asp, which remove phosphorylatively regulable serines). This is joined at its COOH-terminal with the Gly525Arg mutant ligand-binding domain of the mouse estrogen receptor (ERTM), which renders the fusion protein's activity inducible by 4-hydroxytamoxifen, but unresponsive to natural estrogen (17beta-estradiol). (J:92407, J:118584, J:118587, J:118713, J:118714)