Log In|Sign Up
EN
The human hypoxia-inducible factor 1 alpha sequence coding for amino acids 530-652, including the C-terminal portion of the oxygen-dependent degradation domain (ODD) was fused to the firefly luciferase (luc). The fusion protein was preceded by a loxP-flanked PGK-neo cassette and followed by a bovine polyadenylation sequence. The loxP-flanked neomycin was removed by crossing to a cre-expressing mouse line. In the resulting mice, hypoxia results in stabilization of the fusion protein and enhanced fluorescence detectable in hypoxic tissue. (J:118308)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
Show/Hide columns
| Phenotypes |
|---|
References Literature
Title
PMID
Journal
Year
IF
