Tg(Sfpi1,-EGFP)7Dgt

A Sfpi1 mini-gene comprising a 3.4-kb genomic DNA fragment containing the -14-kb upstream regulatory element (URE), a 2.1-kb genomic segment containing the promoter, and a cDNA representing the processed transcript of the gene is followed by an internal ribosome entry site (IRES), the enhanced green fluorescent protein (EGFP) coding sequence, and exon 2, intron 2, exon 3 and the polyadenylation signal of the rabbit beta-globin gene. Reporter expression is detected in a pattern similar to the endogenous gene, with high expression in myeloid cells, and to a lesser extent in B lymphocytes and erythroid cells. Expression is seen in total bone marrow, but is not detected in the thymus (J:117409)