Atf2^{Tg(Gzma-Klra1)7Wum}

A murine granzyme A genomic construct (carrying 11-kb putative promoter and 7-kb structural gene with poly-A addition signal) was used for expression of a Ly49A cDNA. The granzyme A translation start codon was deleted and replaced with a Ly49A cDNA that was inserted into the SmaI site of the modified granzyme A gene. Transgenic mice derived from founder no. 7 expressed Ly49A on nearly all splenic NK1.11 CD32 cells and also expressed Ly49A on approximately one-half of T cells but not on other cell types. Sequence analysis revealed that the Tg construct inserted into exon 5 of the gene for Atf2. Relative to transcription of Atf 2, the Tg construct is directed in the opposite orientation. Analysis of the 3' integration site indicated a fusion of the Tg construct and intron 10. At this end, the Tg construct is oriented in the same direction as Atf 2. PCR of genomic DNA from Tg mice with primers derived from Atf 2 and the transgenic construct confirmed both integration sites into Atf 2. Thus, multiple copies of the Tg construct have inserted into Atf2. Sequence analysis demonstrated that truncated versions of ATF-2 transcripts encoding only dimerization and DNA binding domains were expressed in Tg NK cells. (J:61178, J:127589)