Exon 3 was removed via cre-mediated recombination in ES cells, resulting in a frameshift mutation and a premature translation product containing only the N-terminal 42 residues. Absence of transcript was confirmed by RT-PCR of mutants. (J:117102)
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Exon 3 was removed via cre-mediated recombination in ES cells, resulting in a frameshift mutation and a premature translation product containing only the N-terminal 42 residues. Absence of transcript was confirmed by RT-PCR of mutants. (J:117102)