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The recombined immunoglobulin genes present in the HyHEL10 hybridoma produce antibodies with high affinity for hen egg lysozyme (HEL). The recombined murine Igk variable and joining gene segments present in this hybridoma were placed under the control of a Vkappa22-33 promoter. A floxed neomycin resistance cassette was placed upstream and the entire construct replaced the endogenous Igk-J1 gene segment. Mice were crossed with cre-transgenic mice to removed the floxed neomycin cassette. The transgenic IgK light chain is detectable on some B cells in heterozygote mice. (J:115079)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
