A loxP site was inserted upstream of exon 2. An FRT-flanked puromycin resistance cassette with a 3' loxP site was inserted downstream of exon 4. Exons 2-4 were removed via cre-mediated recombination. Immunoblot failed to detect protein in mutant MEFs. The final allele lacks the puromycin resistance cassette. The floxed allele Cd151tm2Nki was used to generate this allele. (J:114988)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129P2/OlaHsd
Targeted
Insertion, Intragenic deletion
--
1
2
3

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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