A neomycin resistance gene replaced sequence spanning from exon 4 to intron 4, encoding amino acids 138-153. Western blot confirmed that wild-type protein was not detected in mutants. (J:114691)
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A neomycin resistance gene replaced sequence spanning from exon 4 to intron 4, encoding amino acids 138-153. Western blot confirmed that wild-type protein was not detected in mutants. (J:114691)