Tg(CD46)2Gsv

The transgene comprises a partially intact, approximately 420-kb yeast artificial chromosome (YAC) from the Imperial Cancer Research Fund (ICRF, London, UK), designatied AM2: 6C10, that contains the complete human CD46/MCP gene including its 5' and 3' flanking regions. The genome of transgenic mice of line MY II contains 10-12 intact copies of CD46. The expected 4.5 - 4.8-kb transcript, as well as several smaller RNAs, are expressed at high levels in all tissues examined from transgenic mice. Immunohistochemistry likewise detects patterns of expression of human CD46 protein in transgenic mouse tissues similar to those in human tissues. FACS analysis detects CD46 on transgenic spleen cells at approximately 10-fold higher levels than on human splenocytes. (J:113644)