Apob^tm1Mae

Using an 'in step' recombination strategy, the sequence encoding the putative binding domains in exon 26 were replaced with those encoding human HBB peptides. The 5' binding domain (3143LSVKAQYKKNSD3154) was replaced with amino acids 1-12 of the HBBS globin (1VHLTPVEKSAVT12). The 3' binding domain (3356GTSRLMRKRGL3366) was replaced with amino acids 120-131 of HBBA globin (120KEFTPPVQAAYQ131). The reading frame was maintained in the mutant allele, however, the disruption creates a premature stop codon at position 3688. The mutant allele contains 3687 residues, which is 81% of the full length protein. (J:36426)