A targeting vector was designed to replace exon 1, intron 1 and the first 15 bp of exon 2 with cDNA encoding YFP and a floxed PGK-neo cassette. Transient expression of cre recombinase removed the neo cassette. (J:113138)
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A targeting vector was designed to replace exon 1, intron 1 and the first 15 bp of exon 2 with cDNA encoding YFP and a floxed PGK-neo cassette. Transient expression of cre recombinase removed the neo cassette. (J:113138)