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An IRES element, eYFP and a bovine polyA sequence followed by a floxed neomycin resistance cassette were inserted into the end of the coding sequence. Targeting took place in ES cells homozygous for Tg(Prm-cre)70Og, which express Cre recombinase in male germ cells. Cre-mediated excision subsequently removed the neomycin cassette upon transmission from male chimeric mice. The protamine-cre transgene was separated from the knock-in allele by crossing germline male mutant mice with C57BL/6 or BALB/c mice. (J:113265)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
