An FRT-neo-FRT-loxP cassette was inserted in an intron 180bp 5' to the ATG of the Ngn2 gene. Five myc tags were inserted at the N-terminal end of the coding sequence and fused to the full length mouse coding sequence (minus the first codon). A loxP site was located 1.3 kb after the stop codon. (personal communication)
Basic Information
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count