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Homologous recombination was used to introduce the EGFP cDNA into exon 3 in frame and upstream of the stop codon. Western blot demonstrated expression of an EGFP protein as expected. Quantitative mRNA analysis demonstrated that the insertion did not interrupt normal transcription of the locus. (J:111033)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
