A gene trap vector containing a splice acceptor sequence, an internal ribosomal entry site, a selectable colorimetric marker, beta-geo and a polyA sequence was inserted into intron 13 of the locus. RT-PCR confirmed the expression of a fusion transcript in mutant brains, however, the level of expression was significantly lower than in wild-type mice. (J:108880)
Basic Information
Insertion of gene trap vector
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count