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A loxP flanked stop cassette was inserted downstream of the proximal promoter into the 5'UTR. To stop transcripts from the distal promoter, the cassette included a 1.7 kb splice acceptor (SA) sequence from the mouse Engrailed-2 gene. The NLS was placed in-frame between the upstream distal promoter exons and the Betagal gene, and transcription stop elements were included downstream of lacZ to prevent read-through of the cassette. The neo was excised by transient expression of cre recombinase. (The Engrailed2-SA-lacZ inserted sequence is flanked by loxP sites.) (J:106769)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
