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The ~8.1 kb transgene comprises the mouse Zp3 cDNA into which the the enhanced green fluorescent protein (EGFP) cDNA has been inserted downstream of the sequence encoding the 40 amino acid N-terminal signal peptide. Expression of the fusion gene is driven by ~6 kb of the oocyte-specific mouse Zp3 promoter. Ovulated ova and growing oocytes from transgenic mice exhibit EGFP fluorescence throughout the matrix of the zona pellucida; surrounding somatic cells do not fluoresce. Mature oocytes exhibit the strongest signal. Smaller, growing oocytes contain large, circular structures whose boundaries fluoresce and costain with BODIPY-Texas red ceramide. (J:78932, J:86870)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
