The transgene was constructed by joining the coding sequence and SV40 polyadenylation signal from the p-EYFP-Tub vector (Clontech), which encodes a fusion protein composed of enhanced yellow fluorescent protein (EYFP) and a human alpha-tubulin, downstream of the chicken beta-actin promoter followed by a Pgk-puromycin resistance cassette. Mitotic spindles of regenerating hepatocytes from partially hepatectomized mice bearing this transgene fluoresce brightly. (J:100110)
Basic Information
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count