Ednrb^tm1(tTA)Tilg

The majority of the first exon and 230 base pairs of the first intron were replaced with the tTA coding region. The first exon encodes the amino-terminal extracellular domain and the first transmembrane domain of the locus. A neo flanked by two loxP sites was placed downstream of the tTA cassette, putting the transactivator protein under the control of the endogenous promoter. Crossing with mice expressing cre recombinase in the germline removed the floxed neo. (J:58698)