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Two-step homologous recombination was used to delete 70 of the Tcrb locus (475 kb). The first targeting event replaced Dbeta1-Jbeta1 cluster with a loxP flanked neo and subsequent cre expression in the ES cell removed neo leaving a single loxP site. The second targeting event inserted another loxP flanked neo gene into the Vbeta1 exon. Subsequent cre expression in the selected ES clone removed large 475 kb region including the 3' half of the 5'Vbeta region, a central large intron of the locus and the first Dbeta-Jbeta cluster. (J:84291)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
