A neomycin resistance cassette replaced exons 3-5, encompassing the dimerization and DNS-binding domains of the gene. Northern blot and Western blot confirmed absence of transcript and protein, respectively, in mutants. (J:106219)
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A neomycin resistance cassette replaced exons 3-5, encompassing the dimerization and DNS-binding domains of the gene. Northern blot and Western blot confirmed absence of transcript and protein, respectively, in mutants. (J:106219)