Abcc6^tm1Aabb

Exons 16-18, encoding amino acids 647-782 of the transcript and the largest part of the first nucleotide-binding fold (NBF1) including Walker A and B and the signature C motifs, were replaced with a PGK-hygro cassette. RT-PCR failed to detect transcript using primers located within the deleted area. An aberrant transcript that lacked the NBF1 region was detected, however, sequencing revealed that any putative reading frame would be interrupted by several stop codons through the introduction of the hygro cassette. Protein was not detected in mutant liver or kidney samples. (J:105062)