A targeting vector was designed to replace 2.5 kb of the locus, encompassing the first exon and all five downstream exons, with a PGK-neo cassette. Quantitative RT-PCR failed to detect transcript in mutants. (J:104801)
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A targeting vector was designed to replace 2.5 kb of the locus, encompassing the first exon and all five downstream exons, with a PGK-neo cassette. Quantitative RT-PCR failed to detect transcript in mutants. (J:104801)