Tg(Gria2*586N)238.2Rsp

This transgene contains multiple copies of a Gria2(N) minigene which encodes an asparagine (N) at the Q/R site. Specifically, the minigene is constructed from a 6 kb genomic DNA fragment comprising over 5 kb of the upstream region, including the Gria2 promoter, exon 1 and part of intron 1, a 1.1-kb KpnI fragment, itself the fusion of two PCR products connecting the murine intron 1 sequence to rat Gria2 cDNA, plus a fragment of Gria2 flip cDNA, mutated to contain an asparagine (N) codon for the Q/R site. Several lines were made with line L238.2 showing highest expression. (J:51845)