A neomycin resistance gene in opposite orietation replaced part of intron 1, exon 2 and part of intron 2. The absence of mRNA in livers was confirmed by Northern blot. Western blot failed to detect protein in mutants. (J:102729)
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A neomycin resistance gene in opposite orietation replaced part of intron 1, exon 2 and part of intron 2. The absence of mRNA in livers was confirmed by Northern blot. Western blot failed to detect protein in mutants. (J:102729)