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An HSV-tk/beta-actin-neo cassette was inserted into intron 2 using a gene targeting strategy. ES cells expressing both markers were selected. After X-irradiation of properly targeted ES cell lines with 400 rad, loss of tk expression was determined using FIAV. Cells no longer expressing tk were further tested for loss of neomycin expression. SSLP typing of polymorphic microsatellite markers determined that this allele contains a deletion of probably less than 658 kb. FISH confirmed deletion of genomic sequence. (J:102039)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
