Log In|Sign Up
EN
The transgene comprises 6.3 kb of genomic DNA containing the entire mouse Lim2 gene, including 399 bp of 5' flanking DNA, with the G-to-T transversion in exon 2 putatively responsible for the dominant total opacity 3 (To3) phenotype. Dot-blot and Southern blot analyses of genomic DNA from transgenic mice indicate presence of two co-integrated copies. RT-PCR of total mouse lens RNA followed by digestion of the PCR product with BstE II, which cuts within the mutant, but not the wildtype, Lim2 cDNA, revealed a low but reproducible ratio of transgenic to wildtype mRNA in lenses from fetal (E18), weanling (P~20) and adult (P>20) transgenic mice. (J:100078)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
Show/Hide columns
| Phenotypes |
|---|
References Literature
Title
PMID
Journal
Year
IF
