Fzd1^tm1Dgen

A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. RT-PCR analysis detects a high level of gene transcript in lung, kidney, skin, gallbladder, urinary bladder, pituitary gland, adrenal gland, colon, epididymis, seminal vesicle, coagulating gland, prostate gland, ovary, uterus and white fat and low levels in whole brain, cortex, subcortical region, cerebellum, brainstem, olfactory bulb, spinal cord, eye, Harderian glands, heart, liver, pancreas, spleen, thymus, lymph nodes, bone marrow, salivary gland, skeletal muscle, tongue, stomach, small intestine, cecum and testis. Beta-galactosidase expression from this allele is detectable in most organs examined. Most striking expression was detected in the central nervous system, muscularis of the urinary bladder and blood vessels. Staining was observed in different tissue and cell types, including cardiac muscle, epithelium, neurons, cartilage, adipose tissue and blood vessels.