Htr5a^tm1Dgen

A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. Low levels of RNA transcripts are detectable in brain, cortex, subcortical region, cerebellum, brainstem, olfactory bulb, spinal cord, eye, skin, pituitary gland, adrenal gland, ovary, and white fat but not in Harderian glands, heart, lung, liver, pancreas, kidney, spleen, thymus, lymph nodes, bone marrow, gallbladder, urinary bladder, salivary gland, skeletal muscle, tongue, stomach, small intestine, large intestine, cecum, testis, epididymis, seminal vesicle, coagulating gland, prostate gland and uterus. LacZ (beta-galactosidase) expression is detectable in brain and esophagus. In brain, staining is restricted to the cerebellum with Purkinje cells and nuclei in the granular layer displaying expression.