A vector was designed to flank exon 1, containing the ATG, with loxP sites. An FRT and floxed neo was inserted upstream of the exon. Transcript was reduced by 20%, possibly due to transcriptional interference from the neo cassette. (J:101203)
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A vector was designed to flank exon 1, containing the ATG, with loxP sites. An FRT and floxed neo was inserted upstream of the exon. Transcript was reduced by 20%, possibly due to transcriptional interference from the neo cassette. (J:101203)