A vector was designed to flank exon 1, containing the ATG, with loxP sites. An FRT and floxed neo was inserted upstream of the exon. Transcript was reduced by 20%, possibly due to transcriptional interference from the neo cassette. (J:101203)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count