Kras^tm1Bbd

In one homologous recombination event an IRES Beta-geo cassette was inserted into the 3' untranslated region and in a second homologous recombination event a mutation changing glycine codon 12 in exon 1 into a valine (p.G12V) was inserted along with a loxP site flanked transcriptional stop and hygromycin resistance gene cassette. This resulted in bicistronic Cre recombination-dependent expression of the oncogenic Kras allele and Beta-geo. (J:86101)