Gata1^tm8.1Sho

An AvrII-NheI genomic fragment spanning exon 2 and part of exon 3 was amplified. Then a 5' loxP site was introduced to the AvrII site and a neo cassette was inserted into the PstI site in the intron between exons 2 and 3. The neo cassette was later removed by transfecting a GFP-Cre plasmid and then carrying out fluorescence-activated cell sorting for GFP+ cells, leaving exon 2 flanked by loxP sites. (J:99310)