An AvrII-NheI genomic fragment spanning exon 2 and part of exon 3 was amplified. Then a 5' loxP site was introduced to the AvrII site and a neo cassette was inserted into the PstI site in the intron between exons 2 and 3. The neo cassette was later removed by transfecting a GFP-Cre plasmid and then carrying out fluorescence-activated cell sorting for GFP+ cells, leaving exon 2 flanked by loxP sites. (J:99310)
Basic Information
129S1/Sv-Oca2+ Tyr+ Kitl+
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count