Exons 1 and 2 were replaced by a 1.2 kb floxed neomycin resistance cassette. Absence of gene expression was confirmed by RT-PCR, northern blot analysis, and western blot analysis. (J:98905)
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Exons 1 and 2 were replaced by a 1.2 kb floxed neomycin resistance cassette. Absence of gene expression was confirmed by RT-PCR, northern blot analysis, and western blot analysis. (J:98905)