A lacZ reporter gene fused in frame to the initiation codon and a PGK-neo selection cassette replaced the complete ORF. RT-PCR confirmed the complete absence of transcript in null mice. (J:96846)
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A lacZ reporter gene fused in frame to the initiation codon and a PGK-neo selection cassette replaced the complete ORF. RT-PCR confirmed the complete absence of transcript in null mice. (J:96846)