Exons 2-21, encoding amino acids 67-774, were replaced with a lacZ-neomycin cassette. Absence of transcript in mutants was confirmed by Northern blot and RT-PCR. (J:97529)
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Exons 2-21, encoding amino acids 67-774, were replaced with a lacZ-neomycin cassette. Absence of transcript in mutants was confirmed by Northern blot and RT-PCR. (J:97529)