Tg(Igh-VB1-8/Igh-6)2Mjsk

The transgene encodes both a membrane-bound and a secreted form of the same immunoglobulin mu heavy chain. The variable region of the transgene comprises the VDJ moiety from the functionally rearranged Igh gene of the C57BL/6-derived B1-8 hybridoma, containing the V-region segment Vh186.2 and recognizing the hapten (4-hydroxy-3-nitrophenyl)acetyl. This is followed by a J-C intron fragment containing the Igh intronic enhancer and an Igh-6 constant region gene (of haplotype a), including the exons encoding both alternative amino termini. (J:55066)