Adamts5^tm1.1Eam

Exon 3 was flanked with loxP sites to allow disruption of the zinc-binding site and deletion of the conserved "Met-turn." Cre mediated recombination of the flanking loxP sites resulted in the deletion of exon 3 from Adamts5tm1Eam. RT-PCR confirmed absence of exon 3 in mutant spleen and cartilage transcripts. A protein product from the transcript was not found in mutant cartilage. (J:97590)