Sema3b^tm1.1Brd

Exons 1-6, encoding the first 163 amino acids and ~40% of the SEMA domain, were flanked with loxP sites. An FRT-flanked puromycin resistance cassette was inserted into intron 6. ES cells were transfected with Flp recombinase to remove the puro. RT-PCR demonstrated that the single loxP site in intron 6 did not interfere with splicing between exons 6 and 7. (J:97316)