Tg(tetO-EGFP/FADD)1Doi

The transgene encodes a fusion protein consisting of a truncated, dominant-negative mutant Fas-associated death domain protein in which amino acids 1-79, constituting the death effector domain, have been replaced by the full-length enhanced green fluorescent protein. The coding sequence, preceded by a minimal CMV promoter and a rabbit beta-globin intron and followed by the beta-globin polyadenylation signal, resides downstream of seven copies of the tet operator (tetO). TetO, when present in cells also carrying a tetracycline transactivator (tTA) or reverse transactivator (rtTA) transgene, is inactivated or activated, respectively, by the administration of tetracycline. (J:94935, J:95005)