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Exons 2 and 3, encoding the caspase activation and recruitment domain (CARD) were replaced with a lacZ reporter gene and a PGK-neo. Retention of stop codons downstream of the exon 2 splice acceptor site and a consensus Kozak start site for lacZ, as well as the splicing of exon 1 to exon 4 allowed an ORF that included lacZ. Immunoprecipitation and Western blot of mutant spleen cells confirmed production of a protein without the CARD. Beta-galactosidase activity was detected in thymus, spleen and lymph nodes, but negligible in bone marrow of heterozygous mice. (J:95800)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
