A targeting construct was designed to flank exon 5 with loxP sites and insert an FRT-flanked sequence containing a splice acceptor site, stop codons in all three reading frames, and an IRES-gfp-neo. Transcription resulting in the bicistronic mRNA was confirmed by flow cytometry. (J:95651)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count