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A targeting vector was designed to replace exons 1-4, which contain most of the coding sequence, with a neo gene lacking the polyadenlylation sequence. RT-PCR and sequencing revealed that exons 5 and 6 were transcribed after the stop codon of the neo mRNA, as part of the 3' UTR. Absence of protein from exons 5 and 6 was further confirmed by Western blot analysis of kidneys from mutant animals. (J:95282)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
