A floxed neo cassette was inserted downstream of exon 2, and an additional loxP site was inserted downstream of exon 4. Germ line, cre mediated recombination was used to remove the neo cassette leaving exons 2 through 4 floxed.
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count