FRT-flanked PGK-hygro with a loxP site downstream and a lacZ sequence was inserted downstream of the gene in position 3444. A second loxP site was inserted into position 540 in intron 1. RT-PCR and Western blot of mutant MEFs demonstrated the expression of mRNA or protein, respectively. (J:96106)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129P2/OlaHsd
Targeted
Insertion
--
1
--
4

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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