Pank3^Gt(XE134)Byg

The pGT1Lxf gene trap vector contains the Betageo gene, a fusion of the bacterial beta-galactosidase (lacZ) and neomycin phosphotransferase 2 (neor) coding sequences, downstream of a splice acceptor/exon of the engrailed 2 (En2) gene. In this instance the vector inserted correctly into an intron of the mouse gene, so that splicing of the primary transcript of the trapped gene joins its previous exon to the En2 exon. Expression from the endogenous promoter is expected to result in expression of beta-galactosidase in cells where the endogenous gene is normally expressed. (J:94303)