The coding sequence of beta-galactosidase followed by a PGK-neo cassette was inserted in frame downstream of the sequence for residue 180. QRT-PCR and Western blot analysis confirmed the absence of transcription and translation products. (J:111469)
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The coding sequence of beta-galactosidase followed by a PGK-neo cassette was inserted in frame downstream of the sequence for residue 180. QRT-PCR and Western blot analysis confirmed the absence of transcription and translation products. (J:111469)