Dmbt1^tm1Kumc

The first exon and 300 bp of the first intron were replaced by a cassette composed of a GFP cassette inserted downstream of the TATA box in such a way that the GFP start codon replaced that of the endogenous gene as well as a PGK neomycin gene in reverse orientation. Lack of expression was confirmed by QRT-PCR and Western blot analysis. No GFP protein expression or mRNA was detected. (J:132597)