Exons 2-6 of dPRP were replaced with a GFP and a neo gene. Absence of transcription was confirmed by Northern blot analysis, in situ hybridization, and RT-PCR. Absence of protein product was demonstrated by immunohistochemistry. (J:117034)
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Exons 2-6 of dPRP were replaced with a GFP and a neo gene. Absence of transcription was confirmed by Northern blot analysis, in situ hybridization, and RT-PCR. Absence of protein product was demonstrated by immunohistochemistry. (J:117034)