Dnah1^ferf1

This phenotypic mutant was identified in an ENU mutagenesis screen. Targeted exome sequencing and SNP analyses identified a single coding mutation, a T to C mutation located in exon 73 of the Dnah1 gene, resulting in a tyrosine to histidine change at amino acid 3897 (Y3897H). GRCm39 has the T to C mutation resulting in the Y to H substitution at amino acid 3898 (p.Y3898H). The mutation site was further confirmed by genomic PCR and RT-PCR followed by sequencing. This mutation does not result in failure to produce DNAH1 protein or its degradation. (J:308831)