A neomycin resistance gene replaced exon 5 and a stop codon introduced into exon 4, thereby eliminating essential portions of the BIR domain. Immunoblot of mutants indicated successful recombination by a lack of protein. (J:94169)
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A neomycin resistance gene replaced exon 5 and a stop codon introduced into exon 4, thereby eliminating essential portions of the BIR domain. Immunoblot of mutants indicated successful recombination by a lack of protein. (J:94169)